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In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based

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In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Suppliers list
Company Name: Sigma-Aldrich  
Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Products Intro: Product Name:In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
Purity:In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Package:1kit Remarks:TOX7
Company Name: Riedel-de Haen AG  
Tel: 800 558-9160
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Company Name: SIGMA-RBI  
Tel: 800 736 3690 (Orders)
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In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Basic information
Product Name:In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
Synonyms:In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
CAS:
MF:
MW:0
EINECS:
Product Categories:Cell AnalysisCell Signaling and Neuroscience;Cell Biology;Cell Growth and Cell Viability KitsReagents and Supplements;Cell Viability and Proliferation;Kits;Signal Transduction Research Tools
Mol File:Mol File
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Structure
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Chemical Properties
storage temp. −20°C
Safety Information
Hazard Codes Xn,Xi
Risk Statements 22-41-52/53-36
Safety Statements 26-36-61
MSDS Information
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Usage And Synthesis
UsesIn Vitro Toxicology Assay Kit, Lactic Dehydrogenase based has been used:
  • to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay
  • to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells
  • to examine the activity of LDH in RAW 264.7 cells , in LDH cytotoxicity assay in human cell lines
  • for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm.
General DescriptionTox7 is a member of Sigma′s CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays, as well as conventional cytotoxicity resulting from interaction of a test material with the cell.
Biochem/physiol ActionsThe lactate dehydrogenase assay is a means of measuring either the number of cells via total cytoplasmic lactate dehydrogenase (LDH) or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. This method is simple, accurate and yields reproducible results. The assay is based on the reduction of NAD by LDH. The resulting reduced NAD (NADH) is utilized in the stoichiometric conversion of a tetrazolium dye to a soluble, colored formazan derivative..
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Preparation Products And Raw materials
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