| Company Name: |
Sigma-Aldrich
|
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Azurin
CAS:12284-43-4
Purity:from Pseudomonas aeruginosa, lyophilized powder Package:1MG Remarks:A3672-1MG
|
| Company Name: |
Energy Chemical
|
| Tel: |
021-58432009 400-005-6266 |
| Email: |
marketing1@energy-chemical.com |
| Products Intro: |
Product Name:Azurin from Pseudomonas aeruginosa, lyophilized powder
CAS:12284-43-4
Purity:NULL Package:1mg Remarks:NULL
|
| Company Name: |
Riedel-de Haen AG
|
| Tel: |
800 558-9160 |
| Email: |
|
| Products Intro: |
Product Name:Azurin
CAS:12284-43-4
|
| Company Name: |
SIGMA-RBI
|
| Tel: |
800 736 3690 (Orders) |
| Email: |
|
| Products Intro: |
|
|
| | AZURIN Chemical Properties |
| storage temp. | -20°C | | form | lyophilized powder |
| | AZURIN Usage And Synthesis |
| Uses | Azurin has been used:
- in the cytotoxicity and cell viability studies in human osteosarcoma cell line
- for the functionalization of silicon nitride cantilevers for interaction studies
- for coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica
| | Biochem/physiol Actions | Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition. | | Purification Methods | Azurin with A625/280 = 0.56 is purified by gel chromatography on G-25 Sephadex with 5mM phosphate pH 7 buffer as eluent [Cho et al. J Phys Chem 91 3690 1987]. It is a blue Cu protein used in biological electron transport, and its reduced form is obtained by adding a slight excess of Na2S2O4. [See Structure and Bonding Springer Verlag, Berlin 23 1 1975.] |
| | AZURIN Preparation Products And Raw materials |
|