H-THR-PHE-LEU-LEU-ARG-NH2
| 中文名称 | H-THR-PHE-LEU-LEU-ARG-NH2 |
|---|---|
| 中文同义词 | 血酶受体;化合物TFLLR-NH2(2TFA) |
| 英文名称 | TFLLR-NH2 |
| 英文同义词 | H-THR-PHE-LEU-LEU-ARG-NH2;THR-PHE-LEU-LEU-ARG-NH2;THROMBIN RECEPTOR (PAR-1), HYBRIDE HUMAN-XENOPE;TFLLR-AMIDE;TFLLR-NH2;(Thr1)-TRAP-5 amide;TFLLR-NH2 Protease-Activated Receptor 1 (PAR1) Agonist;TFLLRaMide, (Thr1)-TRAP-5 aMide, (Thr1)-PAR-1 (1-5) aMide (huMan) |
| CAS号 | 197794-83-5 |
| 分子式 | C31H53N9O6 |
| 分子量 | 647.81 |
| EINECS号 | |
| 相关类别 | 标准品;Proteinase-activated receptor (PAR);Peptide Receptors |
| Mol文件 | 197794-83-5.mol |
| 结构式 |  |
H-THR-PHE-LEU-LEU-ARG-NH2 性质
| 密度 | 1.31±0.1 g/cm3(Predicted) |
|---|---|
| 储存条件 | Desiccate at -20°C |
| 酸度系数(pKa) | 12.10±0.45(Predicted) |
| 形态 | 粉末 |
| 水溶解性 | Soluble to 1 mg/ml in water |
EC50: 1.9 μM (PAR1)
PAR1 agonists stimulate concentration-dependent increases in [Ca 2+ ]i and in the proportions of neurones. The maximal increase in [Ca 2+ ]i above basal is detected in response to 10 μm TF-NH2(peak 196.5±20.4 nM, n=25) when 50–80% of identified neurones responded. SW620 cells cultured in the supernatant of TFLLR-NH2-activated platelets upregulate E-cadherin expression and downregulate the vimentin expression. In the in vitro platelet culture system, a TFLLR-NH2 dose-dependent increase of secreted TGF-β1 is detected in the supernatant.
Injection of TF-NH2 into the rat paw stimulates a marked and sustained oedema. An NK1R antagonist and ablation of sensory nerves with capsaicin inhibit oedema by 44% at 1 h and completely by 5 h. In wild-type but not PAR1 −/− mice, TF-NH2 stimulates Evans blue extravasation in the bladder, oesophagus, stomach, intestine and pancreas by 2–8 fold. Extravasation in the bladder, oesophagus and stomach is abolished by an NK1R antagonist. TFp-NH2 produces notable contraction at 3-50 μM and relaxation at 0.3-50 μM, in the absence of apamin. The concentration-response curve for TFp-NH2-induced contraction is remarkably shifted left, when the TFp-NH2-induced relaxation is blocked by apamin at 0.1 μM.