| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
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orderCN@merckgroup.com |
| Products Intro: |
Product Name:Anti-CRISPR/Cas9 antibody, Mouse monoclonal
Purity:monoclonal 7A9-3A3, purified from hybridoma cell culture Package:100μL Remarks:SAB4200701
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| | Anti-CRISPR/Cas9 antibody, Mouse monoclonal Basic information |
| | Anti-CRISPR/Cas9 antibody, Mouse monoclonal Chemical Properties |
| storage temp. | -20°C | | form | buffered aqueous solution |
| | Anti-CRISPR/Cas9 antibody, Mouse monoclonal Usage And Synthesis |
| Uses | Monoclonal Anti-CRISPR/CAS9 recognizes over-expression systems containing CAS9 construct. The antibody successfully recognizes mutant Cas9 variants, including nickase Cas9 and dead Cas9 (dCas9). The antibody may be used in various immunochemical techniques including Immunoblotting, Immunofluorescence and Immunoprecipitation. Monoclonal Anti-CRISPR/CAS9 does not cross react with FnCas9 from Francisella novicida bacteria and Cpf1 proteins from Acidaminococcus sp. (strain BV3L6) and Lachnospiraceae bacterium ND2006. | | General Description | CAS9, also known as clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein-9 nuclease , cas5 and csn1, is the signature gene of the type II CRISPR -RuvC (RNase H-like fold) cas system. CAS9 contains 1388 amino acids. This protein is predicted to contain a RuvC/ ribonuclease (RNase) H domain involved in crRNA maturation and McrA/HNH signature domain involved in the DNA degradation step. | | Biochem/physiol Actions | CAS9 plays a vital role in plasmid DNA interference. It is the only cas protein needed to deliver resistance against foreign DNA. CAS9 stimulates both RNA-guided genome editing and gene regulation in various organisms, but it can facilitate only one activity at a time within any given cell. |
| | Anti-CRISPR/Cas9 antibody, Mouse monoclonal Preparation Products And Raw materials |
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