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| | DC-CHOL Basic information |
| Product Name: | DC-CHOL | | Synonyms: | cholesteryl 3B-(N-(dimethylaminoethyl)*carbamate);3β-[n-(dimethylaminoethane) carbamoyl] cholesterol;CHOLESTERYL N-[DIMETHYL-AMINOETHYL]CARBAMATE HYDROCHLORIDE;DC-CHOL;DC-CHOLESTEROL;cholesteryl 3beta-N-(dimethylaminomethyl)carbamate HCl;3β-[N-(Dimethylaminoethane)carbamoyl]cholesterol, DC-Chol;O-[(N,N-Dimethylaminoethyl)-carbamoyl]cholesterol hydrochloride | | CAS: | 166023-21-8 | | MF: | C29H51ClN2O2 | | MW: | 495.18 | | EINECS: | | | Product Categories: | | | Mol File: | 166023-21-8.mol |  |
| | DC-CHOL Chemical Properties |
| storage temp. | -20°C | | solubility | DMSO: > 5mg/mL; Methanol: > 10 mg/mL | | form | A crystalline solid | | color | White to light yellow |
| | DC-CHOL Usage And Synthesis |
| Description | DC-Chol is a cationic cholesterol derivative. DC-Chol, as a component of lipoplexes with DOPE , has been used for transfection of mRNA into A549 cells without affecting cell viability. Incubation of DC-chol/DOPE liposomes or lipoplexes with human whole blood has no effect on neutrophil elastase or β-thromboglobulin levels or the number of platelets and red and white blood cells, indicating hemocompatibility. DC-Chol has also been widely used in the synthesis of liposomes for the delivery of siRNA, DNA, and chemotherapeutic agents into cells and mice. | | Uses | This cationic cholesterol derivative forms liposomes with L-α-phosphatidylethanolamine, dioleoyl, that efficiently transfect mammalian cell lines. | | Uses | DC-Cholesterol HCl is suitable:
- for the preparation of ultrasound cationic microbubbles
- to prepare liposomes
- for the preparation of L-glutathione (GSH), loaded proliposomes
| | General Description | DC-Cholesterol HCl is a sterol-based transfection reagent. | | Biochem/physiol Actions | DC-Cholesterol is an effective transfection agent and a liposome reagent. Liposome of DC-Cholesterol with dioleoylphosphatidylethanolamine (DOPE) are efficient for DNA transfection. It imparts positive surface charge to the microbubbles for ultra sound based transfection of clustered regularly interspaced short palindromic repeats (CRISPRs). | | storage | Store at -20°C |
| | DC-CHOL Preparation Products And Raw materials |
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