Bromothymol Blue NEW

Price	$5	$0.1
Package	1KG	1000KG

Min. Order:	1KG
Supply Ability:	g-kg-tons, free sample is available
Update Time:	2024-04-05

Product Details

Product Name: Bromothymol Blue	CAS No.: 76-59-5
Min. Order: 1KG	Purity: 98%
Supply Ability: g-kg-tons, free sample is available	Release date: 2024/04/05
Lead time: In stock, ready for shipment	Packaging: bag/bottle/drum/IBC
Delivery: By express, by air, by sea	Origin: Manufacturer, advantage product
COA, MSDS: Available, contact us for details	Name: Tina

1. Materials information

Name	bromothymol blue
Synonym	More Synonyms

Description	Bromothymol Blue is a pH indicator.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies[2].
Kinase Assay	For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress(DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KPi, pH 7.0, 2 mM MgCl2, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KPi, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KPi, pH 7.0, 2 mM MgCl2, 25 μg/mL BTB and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation[1].
References	[1]. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80. [2]. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul - Sep;48(3):397-402.

Density	1.5±0.1 g/cm3
Boiling Point	614.3±55.0 °C at 760 mmHg
Melting Point	204 °C
Molecular Formula	C27H28Br2O5S
Molecular Weight	624.381
Flash Point	325.3±31.5 °C
Exact Mass	622.002380
PSA	92.21000
LogP	8.60
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.634
Storage condition	Store at RT.
Stability	Stable. Incompatible with strong oxidizing agents.
Water Solubility	slightly soluble

Bromothymol blue MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SJ7450000

CHEMICAL NAME :
Phenol, 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bis(2-bromo-3-met hyl-6-(1-methylethyl) -, S,S-dioxide

CAS REGISTRY NUMBER :
76-59-5

BEILSTEIN REFERENCE NO. :
0373934

LAST UPDATED :
199701

DATA ITEMS CITED :
5

MOLECULAR FORMULA :
C27-H28-Br2-O5-S

MOLECULAR WEIGHT :
624.43

Symbol	GHS02
Signal Word	Warning
Hazard Statements	H226
Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xn
Risk Phrases	R10
Safety Phrases	S24/25-S39-S26
RIDADR	NONH for all modes of transport
RTECS	SJ450000
Packaging Group	III
HS Code	29349990

Thymol blue

CAS#:76-61-9

Bromothymol blue

CAS#:76-59-5

Literature: J. Washington Acad., vol. 6, p. 482 Chem. Zentralbl., , vol. 87, # II p. 1068 J. Washington Acad., vol. 5, p. 616 Chem. Zentralbl., , vol. 87, # I p. 175

Thymol

CAS#:89-83-8

Bromothymol blue

CAS#:76-59-5

Literature: J. Washington Acad., vol. 6, p. 482 Chem. Zentralbl., , vol. 87, # II p. 1068

Bromine

CAS#:7726-95-6

Thymol blue

CAS#:76-61-9

acetic acid

CAS#:64-19-7

Bromothymol blue

CAS#:76-59-5

Literature: J. Washington Acad., vol. 6, p. 482 Chem. Zentralbl., , vol. 87, # II p. 1068 J. Washington Acad., vol. 5, p. 616 Chem. Zentralbl., , vol. 87, # I p. 175

Precursor 4
CAS#:76-61-9 Thymol blue CAS#:89-83-8 Thymol CAS#:7726-95-6 Bromine CAS#:64-19-7 acetic acid

2. Packaging of materials

For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.
For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product.

Or smaller package 1kg/bottle, 10kgs/bottle as request.

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3. Shipping & Delivery

By Express

Provide door to door service

Suitable for goods under 50kg

Delivery: 3-7 days

Cost: low cost

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By Air

Provide airport to airport service

Suitable for goods over 50kg

Delivery: 3-14 days

Cost: high cost

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By Sea

Provide seaport to seaport service

Suitable for goods over 100kg

Delivery: 2-45 days

Cost: low cost

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4. Contact information

For more details, pls contact us freely.

Email address: Tina@fdachem.com

Mob: 86 15225627621

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Company Profile Introduction

Henan Fengda Chemical Co., Ltd. is located in the High-tech Development Zone of Henan Province. Specializing in the production and sales of various fine chemical products required for industrial production, including chemical raw materials, organic raw materials, petrochemicals, chemical reagents, solvents, catalysts, and additives, etc.

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