Concanavalin a (jack bean)

English Name: concanavalin A (Jack bean); Con A

Other names: concanavalin A; concanavalin A; concanavalin A; concanavalin A; soybean globulin A

CAS No.: 11028-71-0

Level: affinity chromatography pure

Hemagglutination activity: 1.95mg/ml (when con a concentration is 1.95mg/ml, it can make the RBC agglutinate, and its agglutination ability can be inhibited by D-glucose (d-glc), D-mannose (d-man), N-acetylglucosamine (n-glcnac) and sucrose)

Lymphocyte transformation rate: > 75%

Properties: affinity chromatography purification of freeze-dried salt-free freeze-dried powder. It is soluble in water, normal saline or buffer solution. The solution is clear and insoluble. A plant agglutinin extracted from the seeds of concanavalis and peas. It is a tetramer globulin with molecular weight of 102000. Each subunit contains 237 amino acid residues, with a molecular weight of 25500. It binds one Ca2 + and one Mn2 +, and contains one sugar binding site. It can specifically bind to α - mannose and α - glucose (on the glycoprotein of cell membrane). The binding site is required to be C-3 / C-4 and C-6 in the six membered ring of α - D-glucopyranose or α - D-glucopyranose. It has the highest affinity with mannose and mannose linked by α - 1,2 glycoside bond

Purpose: biochemical research. It is Mito gen, which can stimulate T lymphocytes, agglutinate erythrocytes and animal sperm, inhibit tumor cell movement, and prolong the survival time of xenotransplantation. An important biochemical and immune research reagent.

Storage: 2-8 ºC