L-Glutathione reduced

L-Glutathione reduced

Glutathione is a tripeptide cysteine-glycine-glutamic acid which exists in cells in the reduced form (this product) or oxidised form. Reduced glutathione (GSH) is an antioxidant protecting cell components from endogenous and exogeneous reactive oxygen and nitrogen species. Reduced glutathione has also been intensely used in the affinity purification of proteins with glutathione S-transferase (GST) tag. In the protein purification process, glutathione is used for the elution of GST-fused recombinant proteins from a glutathione-immobilised resins. In the elution buffers, reduced glutathione is typically used in the 10 – 40 mM concentration range.

Name L-Glutathione reduced

Synonyms: 5-L-Glutamyl-L-cysteinylglycine;GSH;gamma-L-Glutamyl-L-cysteinylglycine

Chemical Name: L-γ-glutamyl-L-cysteinyl-glycine

CAS Number: 70-18-8

EC Number: 200-725-4

Molecular Formula C10H17N3O6S

Molecular Weight: 307.32

Purity: 98%

Appearance: White powder

Use: anti-aging

Application Dietary supplement, Pharmaceutical ,IVD

technique(s) cell culture | mammalian: suitable

Testing Document Available

Water/Solubility (25°C) H2O: 50 mg/mL

mp 192-195 °C (dec.) (lit.)

Ethanol/Solubility (25°C) Insoluble

storage temp.  : Inert atmosphere,2-8°C

Package 10gram/bag ,50gram/bag , 1KG/bag, 25KG/drum

solubility water: 1 mg/mL

DMF: 10 mg/mL

ethanol: 5 mg/mL

DESCRIPTION

Amino Acid Sequence

γ-Glu-Cys-Gly

Application

May be used at 5-10 mM to elute glutathione S-transferase (GST) from glutathione agarose.

Biochem/physiol Actions

Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.