NHS N-Hydroxysuccinimide

Description of N-Hydroxysuccinimide from Watson Biotechnology Co., Ltd

Specifications(COA) of N-Hydroxysuccinimide CAS 6066-82-6 use for Protein and Peptide Conjugation

N-Hydroxysuccinimide (NHS) is commonly used in various biochemical applications, particularly in the field of protein and peptide chemistry. Here are some of its key applications:

### 1. **Protein and Peptide Conjugation:**

   - **Amine-Reactive Crosslinking:** NHS is often used in conjunction with carbodiimides like EDC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) to activate carboxyl groups on proteins or peptides. This activation allows the carboxyl group to react with amines, forming stable amide bonds, which is essential in conjugating proteins or peptides to other molecules, such as fluorophores, drugs, or solid supports.

   - **Bioconjugation:** NHS esters are frequently used to link biomolecules like antibodies or enzymes to solid surfaces or other molecules, enabling the creation of bioactive materials for diagnostics, therapeutic applications, or research.

### 2. **Labeling and Detection:**

   - **Fluorescent Labeling:** NHS esters of fluorescent dyes are widely used to label proteins, peptides, and other biomolecules. The NHS group reacts with primary amines (e.g., lysine residues), allowing the dye to be covalently attached to the target molecule, facilitating detection and imaging in various assays.

   - **Radioactive Labeling:** NHS esters are also employed to attach radioactive isotopes to biomolecules, useful in imaging or tracing studies in biomedical research.

### 3. **Solid-Phase Synthesis:**

   - **Peptide Synthesis:** In solid-phase peptide synthesis, NHS esters are used to introduce protected amino acids into the growing peptide chain. The NHS ester reacts with the amino group of the previous amino acid, forming a peptide bond.

   - **Immobilization of Biomolecules:** NHS is used to attach biomolecules to solid supports (such as beads or plates) in assays like ELISA (Enzyme-Linked Immunosorbent Assay) or affinity chromatography. This immobilization is crucial for various diagnostic and research applications.

### 4. **Drug Delivery and Therapeutics:**

   - **Prodrug Formation:** NHS esters can be used in the design of prodrugs, where an active drug is linked to a carrier molecule via an NHS ester. This approach can improve the solubility, stability, or targeting of the drug.

   - **PEGylation:** NHS esters are used in the process of PEGylation, where polyethylene glycol (PEG) is attached to proteins, peptides, or drugs to increase their solubility, stability, and half-life in the bloodstream.

### 5. **Surface Modification:**

   - **Material Surface Functionalization:** NHS esters are employed to modify the surface of materials like nanoparticles, polymers, or medical devices by attaching specific biomolecules, improving their biocompatibility or targeting capabilities.

N-Hydroxysuccinimide is a versatile reagent in the field of bioconjugation, enabling the development of advanced biomaterials, drug delivery systems, and diagnostic tools.

Founded in 2006 and headquartered in Wuhan, Hubei, China, Watson Biotechnology Co., Ltd is a leading manufacturing powerhouse specializing in the production of Active Pharmaceutical Ingredients (APIs) and Intermediates, Food and Feed Additives, Agricultural and Aquaculture chemicals, and Fine Chemicals. As a dedicated factory, our operations are driven by a commitment to excellence and innovation, ensuring that our products meet the highest standards of quality and compliance.

With a robust team of over 100 skilled professionals, Watson Biotechnology has established itself as a trusted name in the industry. Our products are meticulously crafted to align with international standards such as USP (United States Pharmacopeia), EP (European Pharmacopoeia), and BP (British Pharmacopoeia), guaranteeing reliability and efficacy.

At Watson Biotechnology, we pride ourselves on our cutting-edge facilities and state-of-the-art technology, which enable us to deliver superior products that cater to a diverse range of needs across various sectors. Our dedication to continuous improvement and customer satisfaction drives us to maintain stringent quality control processes and foster an environment of innovation and growth.

Watson Biotechnology Co., Ltd is a professional manufacturer which provide N-Hydroxysuccinimide with good quality and best price, welcome to contact us to get COA/TDS/MSDS of N-Hydroxysuccinimide.