Anti-B7-H3 antibodyAnti-B7-H3 抗体
PRODUCT DETAILSProduct Name Anti-B7-H3 antibodySpecies Reactivity Human, PrimateSource Recombinant chimeric antibody (ref: mouse hybridoma Clone # 3G3)Isotype IgG1-kappa (human)Expression System HEK 293 cellPurification Protein A purifiedLabel UnconjugatedFormulation Lyophilized from a 0.2 μm filtered solution in PBS with TrehaloseReconstitution Centrifuge vial prior to opening vial. Add 0.5 mL sterile water or 0.01M PBS (pH7.2) at 0.4 mg/mL. Gently pipet solution down the sides of the vial.DO NOT VORTEX.Please note: For research use only. Not for diagnostic or therapeutic use.SHIPPING & STROAGE Shipping Lyophilized product is shipped at ambient temperature.Storage Upon receipt, store immediately at -20 to -70°C:12 months from date of receipt. After reconstitution under sterile conditions: 2 to 8 °C or 1 month or -20 to -70°C for 6 months.Avoid freeze-thaw cycles.APPLICATIONItem Description Data ExamplesImmunoassay Ligand Biding- Sandwich ELISA
In a sandwich ELISA, a concentration of 1 μg/mL (100 μL/well) of recombinant human 4Ig-B7-H3 (Cat.#: 0721141) will be detected by the Anti-B7H3 Ab (Cat.#:0731074) when used with goat anti-human IgG(H+L) as the secondary antibody. The EC50 is 51 ng/mL. Please note: Optimal concentrations should be determined by each application for each lab.
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