双解丝蛋白1(TWF1)重组蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Twinfilin 1
Gene Names: TWF1
Size: 100µg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: Actin-binding protein involved in motile and morphological processes. Inhibits
actin polymerization, likely by sequestering G-actin. By capping the barbed ends of
filaments, it also regulates motility. Seems to play an important role in clathrin-mediated
endocytosis and distribution of endocytic organelles
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Cytoplasm › cytoskeleton
Tissue Specificity: Specificity: Specificity: Specificity: Expressed at high levels in the colon, testis, ovary, prostate and
lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~Ser252 (Accession # Q12792), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 30.26 kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 30.26 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGS-MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVIG SYSQPSDSWD KDYDSFVLPL
LEDKQPCYIL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE VFGTVKEDVS
LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVGVDT KHQTLQGVAF PISREAFQAL EKLNNRQLNY
VQLEIDIKNE IIILANTTNT ELKDLPKRIP KDSARYHFFL YKHSHEGDYL ES
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Beeler JF, et al.(1994) Mol Cell Biol 14 (2): 982-988.
2. Rush J., et al. (2005) Nat. Biotechnol. 23:94-101. 3. Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162. 4. Palmgren S, et al.(2002) J. Cell. Sci. 115 (Pt 5): 881-886.
5. Vartiainen MK, et al.(2003) J. Biol. Chem. 278 (36): 34347-34355.
6. Moseley,J.B. et al. (2006) J Cell Sci. 119 (Pt 8):1547-1557.
7. Hassel S, et al. (2004) Proteomics 4 (5): 1346-1358.
8. Beeler J.F., et al. (1994) Mol. Cell. Biol. 14:982-988.
关键字： A6;PTK9;Actin;双解丝蛋白1(TWF1)重组蛋白;
上海博尔森生物科技有限公司成立于2020年09月02日，注册地位于上海市松江区石湖荡镇石湖新路95号。
上海博尔森生物科技有限公司虽然年轻，但朝气蓬勃，志向远大； 更是一家专注于生命科学与生物技术领域，集产品研发、生产、销售为一体的高科技公司，并立志以竞争力的价格向客户输送高质量的生物试剂。主要研究领域是生命科学，包括分子生物学、细胞生物学、免疫学试剂、重组蛋白，抗体，实验技术服务等多个应用领域。主要产品有：Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白，蛋白定制，细胞系，原代细胞，常用实验试剂，染色液等科研产品。