This protocol details the labeling procedure for proteins of molecular weight (MW) > 40000 Daltons using MSD® SULF0-
TAG NHS Ester. The straightforward procedure involves a buffer exchange (if necessary), an incubation step for 2 hours,
and the use of a spin column to quickly isolate the labeled protein. MSD SULFO-TAG NHS-Ester (Figure 1) is an aminereactive,
N-hydroxysuccinimide ester which readily couples to primary amine groups of proteins under mildly basic conditions
to form a stable amide bond.
MSD SULFO-TAG™conjugates are stable and may be used at low concentrations. These features minimize time, cost, and
labor as large batches of a stable conjugate can be prepared, validated, and used for long periods of time. Its excellent performance
characteristics and simple labeling procedure make MSD SULFO-TAG NHS-Ester the product of choice for molecules
that contain primary amines (e.g. lysine-containing proteins). MSD SULFO-TAG offers low non-specific binding, resulting
in highly sensitive detection when used in conjunction with MSD's SECTOR® instruments and SECTOR PR® readers.
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