骨粘连蛋白(ON)重组蛋白
[ PROPERTIES ]
Residues: Ala18~Ile303 (Accession # P09486),
with two N-terminal Tags, His-tag and T7-tag.
Host: E. coli
Subcellular Location: Secreted, extracellular space,
extracellular matrix, basement membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.0
Predicted Molecular Mass: 36.2kDa
Accurate Molecular Mass: 48kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- APQ QEALPDETEV VEETVAEVTE
VSV GA NPV QV EVGE F D DGAE ETE EE VVAE N PC QN H HCK H G KV CE L DE N N T
PMCVCQDPTS CPAPIGEFEK VCSNDNKTFD SSCHFFATKC TLEGTKKGHK LHLDYIGPCK
YIPPCLDSEL TEFPLRMRDW LKNVLVTLYE RDEDNNLLTE KQKLRVKKIH ENEKRLEAGD
HPVELLARDF EKNYNMYIFP VHWQFGQLDQ HPIDGYLSHT ELAPLRAPLI PMEHCTTRFF
ETCDLDNDKY IALDEWAGCF GIKQKDIDKD LVI
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
关键字： SPARC;BM-40;Secreted ;骨粘连蛋白(ON)重组蛋白;
上海博尔森生物科技有限公司成立于2020年09月02日，注册地位于上海市松江区石湖荡镇石湖新路95号。
上海博尔森生物科技有限公司虽然年轻，但朝气蓬勃，志向远大； 更是一家专注于生命科学与生物技术领域，集产品研发、生产、销售为一体的高科技公司，并立志以竞争力的价格向客户输送高质量的生物试剂。主要研究领域是生命科学，包括分子生物学、细胞生物学、免疫学试剂、重组蛋白，抗体，实验技术服务等多个应用领域。主要产品有：Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白，蛋白定制，细胞系，原代细胞，常用实验试剂，染色液等科研产品。