心肌营养素1(CT1)重组蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Cardiotrophin 1
Synonyms: Synonyms: Synonyms: Synonyms: CT1
Size: 100 µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~Ala201 (Accession # Q16619), with one N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >95%, 22.7 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 22.7 kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 8.7
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS, PH7.2-PH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months .
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with one N-terminal His-tag. Their sequence is listed below.
MGHHHHHHSGSEF MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG
LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP
RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Pennica D., et al. (1996) Cytokine. 8(3):183-9.
2. Jougasaki M. (2010) Adv Clin Chem. 52:41-76.
3. Latchman DS. (2000) Pharmacol Ther. 85(1):29-37.
4. Railson JE., et al. (2002) Cytokine. 17(5):243-53.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
关键字: CTF1;Cardiotrophin;CT1;心肌营养素1(CT1)重组蛋白;
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上海博尔森生物科技有限公司虽然年轻,但朝气蓬勃,志向远大; 更是一家专注于生命科学与生物技术领域,集产品研发、生产、销售为一体的高科技公司,并立志以竞争力的价格向客户输送高质量的生物试剂。主要研究领域是生命科学,包括分子生物学、细胞生物学、免疫学试剂、重组蛋白,抗体,实验技术服务等多个应用领域。主要产品有:Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白,蛋白定制,细胞系,原代细胞,常用实验试剂,染色液等科研产品。