毒蕈碱型胆碱受体M1(CHRM1)重组蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Cholinergic Receptor, Muscarinic 1
Synonyms: Synonyms: Synonyms: Synonyms: CHRM1, Chrm-1
Species: Species: Species: Species: Rat
Size: 100µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cell membrane; Multi-pass
membrane protein. Cell junction, synapse,
postsynaptic cell membrane.
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Arg210~Ala363 (Accession # P08482),
with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 24kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 0.1% Sarcosyl, 5% sucrose.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 18.3kDa
Rat CHRM1 kDa9466.24533262014.415% SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE
SDS-PAGE is a technique that separates proteins based on size. However, the actual band size
observed may differ from the predicted; the common factors may include three terms:
1. Alternative splicing (splice variants).
2. 2. The composition of amino acids may give the protein the different relative charge.
3. Polymerization of the target protein.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.1
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-R IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG
SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV
VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA
KRKTFSLVKE KKA
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Takamori M., et al. (2007) Eur. J. Neurol. 14:1230-1235.
2. Maeda Y., et al. (2006) Am. J. Respir. Crit. Care Med. 174:1119-1124.
3. Santafe M.M., et al. (2003) Eur. J. Neurosci. 17:119-127.
4. Anagnostaras S.G., et al. (2003) Nat. Neurosci. 6:51-58.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
关键字： CHR-M1;HM1;M1;毒蕈碱型胆碱受体M1(CHRM1)重组蛋;
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上海博尔森生物科技有限公司虽然年轻，但朝气蓬勃，志向远大； 更是一家专注于生命科学与生物技术领域，集产品研发、生产、销售为一体的高科技公司，并立志以竞争力的价格向客户输送高质量的生物试剂。主要研究领域是生命科学，包括分子生物学、细胞生物学、免疫学试剂、重组蛋白，抗体，实验技术服务等多个应用领域。主要产品有：Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白，蛋白定制，细胞系，原代细胞，常用实验试剂，染色液等科研产品。