氯化物细胞内通道蛋白1(CLIC1)重组蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Lys79~Lys241
Tags: N-terminal His-Tag
Tissue Specificity: Heart, Liver, Kidney, Pancreas. Subcellular Location: Nucleus. Nucleus membrane; Single-pass membrane
protein Probable. Cytoplasm. Cell membrane. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM
EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.8
Predicted Molecular Mass: 21.9kDa
Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of
0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed. The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. Gene Sequencing (Extract)
Figure 2. SDS-PAGE
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关键字： G6;NCC27; p64CLCP;氯化物细胞内通道蛋白1(CLIC1)重组;
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上海博尔森生物科技有限公司虽然年轻，但朝气蓬勃，志向远大； 更是一家专注于生命科学与生物技术领域，集产品研发、生产、销售为一体的高科技公司，并立志以竞争力的价格向客户输送高质量的生物试剂。主要研究领域是生命科学，包括分子生物学、细胞生物学、免疫学试剂、重组蛋白，抗体，实验技术服务等多个应用领域。主要产品有：Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白，蛋白定制，细胞系，原代细胞，常用实验试剂，染色液等科研产品。