丝裂原激活蛋白激酶激酶4(MAP2K4)重组蛋白
[ PROPERTIES ]
Residues: Val137~Ser394
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P45985
Host: E. coli
Subcellular Location: Cytoplasm. Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 61.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL MSTSFDKFYK
YVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS GNIKLCDFGI
SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL ATGRFPYPKW
NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK DESKRPKYKELLKHPFILMY
EERAVEVACY VCKILDQMPA TPSS
[ REFERENCES ]
1. Derijard B., et al. (1995) Science 267:682-685.
2. Lin A., et al. (1995) Science 268:286-290.
3. Su G.H., et al. (1998) Cancer Res. 58:2339-2342.
4. Tibbles L.A., et al. (1996) EMBO J. 15:7026-7035.
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关键字： JNKK;JNKK1;MAPKK4;丝裂原激活蛋白激酶激酶4(MAP2K4);
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上海博尔森生物科技有限公司虽然年轻，但朝气蓬勃，志向远大； 更是一家专注于生命科学与生物技术领域，集产品研发、生产、销售为一体的高科技公司，并立志以竞争力的价格向客户输送高质量的生物试剂。主要研究领域是生命科学，包括分子生物学、细胞生物学、免疫学试剂、重组蛋白，抗体，实验技术服务等多个应用领域。主要产品有：Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白，蛋白定制，细胞系，原代细胞，常用实验试剂，染色液等科研产品。