近红外荧光染料IRDye 680LT NHS Ester/Maleimide/Carboxylic acid

IRDye 680LT Infrared Dyes

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes, such as Alexa Fluor® 680. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging systems with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively. IRDye 680LT exhibits low background at near-infrared wavelengths resulting in higher signal-to-noise ratios.

IRDye 680LT dye conjugates can be used for quantitative Western blotting and fluorescence microscopy. When using IRDye 680LT secondary antibodies for Western blot detection, it is critical to follow the recommendations in the pack insert. Dilution optimization may be required to achieve best results.

IRDye 680LT NHS Ester

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

Chemical Formula: C54H52N3Na3O22S6

Molecular Weight: 1402.32 g/mol

Exact Mass: 1401.09

IRDye 680LT Maleimide

IRDye 680LT Maleimide will label molecules with free sulfhydryl (–SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

Chemical Formula: C56H55N5Na5O21S6

Molecular Weight: 1427.37 g/mol

Exact Mass: 1426.12

Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.

IRDye 680LT Carboxylic acid

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes, such as Alexa Fluor® 680. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging systems with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively. IRDye 680LT exhibits low background at near-infrared wavelengths resulting in higher signal-to-noise ratios.

IRDye 680LT dye conjugates can be used for quantitative Western blotting and fluorescence microscopy. When using IRDye 680LT secondary antibodies for Western blot detection, it is critical to follow the recommendations in the pack insert. Dilution optimization may be required to achieve best results.

　　IRDye 680LT 红外染料

　　IRDye 680LT 染料易溶于水，比许多其他 700 nm 近红外染料(如 Alexa Fluor® 680)更亮且更耐光。IRDye 680LT 的光谱适合用于具有以下的 LI-COR 成像系统水溶液和甲醇中的大吸光度和发射大值分别为 676 和 693 nm。 IRDye 680LT 在近红外波长下表现出低背景，从而导致更高的信噪比。

　　IRDye 680LT 染料偶联物可用于定量蛋白质印迹和荧光显微镜检查。使用 IRDye 680LT 二抗进行蛋白质印迹检测时，遵循包装插页中的建议至关重要。可能需要稀释优化以获得佳结果。

　　IRDye 680LT NHS 酯

　　LI-COR Biosciences 的 IRDye 红外染料可供研究人员使用近红外 (NIR) 荧光开发应用。

　　标准 NHS 酯化学用于生产用 LI-COR IRDye 红外染料标记的定制探针。 NHS 酯反应基团提供了标记伯胺和仲胺的功能，例如蛋白质中的赖氨酸残基。

　　化学式：C54H52N3Na3O22S6

　　分子量：1402.32 g/mol

　　质量：1401.09

　　IRDye 680LT 马来酰亚胺

　　IRDye 680LT 马来酰亚胺将标记具有游离巯基 (-SH) 基团的分子，例如蛋白质中的半胱氨酸残基。缀合可以在生理pH下进行。

　　化学式：C56H55N5Na5O21S6

　　分子量：1427.37 g/mol

　　质量：1426.12

　　注意：IRDye 680LT 染料产品不应用于小动物体内成像或 In-Cell Western™ 检测。较高水平的荧光强度会产生高背景，使其不利于在这些应用中使用。我们建议在这些应用中使用 IRDye 680RD 染料产品。

　　IRDye 680LT 羧酸

　　IRDye 680LT 染料易溶于水，比许多其他 700 nm 近红外染料(如 Alexa Fluor® 680)更亮且更耐光。IRDye 680LT 的光谱适合用于具有以下的 LI-COR 成像系统水溶液和甲醇中的大吸光度和发射大值分别为 676 和 693 nm。 IRDye 680LT 在近红外波长下表现出低背景，从而导致更高的信噪比。

　　IRDye 680LT 染料偶联物可用于定量蛋白质印迹和荧光显微镜检查。使用 IRDye 680LT 二抗进行蛋白质印迹检测时，遵循包装插页中的建议至关重要。可能需要稀释优化以获得佳结果。

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IRDye 800CW荧光标记麦芽糖醇Maltitol

IRDye 800CW荧光标记麦芽酮糖 Maltulose Monohydrate

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本文由QiyueLh整理，2022年6月1日