IRDye 800CW Streptavidin
IRDye 800CW 链霉亲和素
IRDye 800CW 链霉亲和素以液体形式提供,缓冲液中含有 10 mM 磷酸盐、183 mM NaCl、2.7 nM KCl,pH 7.4,叠氮化钠 0.005% (w/v) 。
要使用,请在使用前短暂离心以消除可能在溶液中形成的聚集体。这将减少非特异性背景染色。对于大多数应用,0.2 到 1.0 µg/ml(1:1,000 到 1:5,000)的浓度通常是令人满意的;但是,可能需要根据经验确定适当的稀释度。
IRDye 800CW Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
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