中文名称

RNA /蛋白质纯化大提试剂盒（提取试剂盒）

英文名字

RNA/Protein Purification Plus Kit

供应商

Norgen Biotek

产品货号

NGB-48200

背景资料

RNA /蛋白质纯化大提试剂盒（提取试剂盒）产品的背景资料详情查看Norgen Biotek品牌提供的产品说明书

产品描述

This kit provides a rapid, single column method for the isolation and purification of total RNA (including miRNA) and proteins sequentially from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are both column purified in under 25 minutes using a single column.Purified RNA is of a high quality and yield, and is suitable for NGS, RT-qPCR and microarrays.This kit eliminates DNA efficiently using a gDNA removal column.Proteins are eluted in buffer and are ready for downstream applications such as Western Blots, Mass Spec and some ELISA applications. The proteins will not require precipitation, resuspending of pellets, or any further cleaning.This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNAand proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

产品特点

Sequentially purify total RNA and total proteins from a single sample; Kit includes a gDNA elimination column; No sample splitting required; No phenol step required for efficient isolation; Ideal for small or difficult to obtain samples; Purify RNA and proteins from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants; Rapid and efficient spin column procedure.

保存建议

溶液密封室温保存，未开封至少保存1年。具体保存情况收到产品后请按说明书存放。

其他

Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。 目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。