中文名称

唾液外泌体纯化试剂盒（提取试剂盒）

英文名字

Saliva Exosome Purification Kit

供应商

Norgen Biotek

产品货号

NGB-65300

背景资料

唾液外泌体纯化试剂盒（提取试剂盒）产品的背景资料详情查看Norgen Biotek品牌提供的产品说明书

产品描述

Exosomes are 40 - 150 nm membrane vesicles which are secreted by most cell types. Exosomes can be found in plasma, serum, saliva, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes and other extracellular vesicles (EVs) which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNA may serve as biomarkers for various diseases including cancer. Another subset of RNA that is found in saliva is the free-circulating RNA (fc-RNA). These fc-RNA are usually protein-bound RNA that are leaked from cells either during apoptosis or necrosis. As the RNA molecules encapsulated within exosomes or bound to proteins (fc-RNA) are protected from degradation by RNAses, they can be efficiently recovered from biological fluids such as saliva. In general, these two RNA groups contain valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status.Norgen’s Saliva Exosome Purification Kit allows for the purification of saliva exosomes from 500 μL to 2 mL of fresh saliva samples or saliva samples collected using Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). This kit also allows for the purification of intact extracellular vesicles (EVs) from different saliva sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-bound proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

产品特点

Purification and enrichment of intact saliva exosomes;Versatile sample input ranging from 0.5 mL to 2 mL;Process fresh saliva or saliva collected with Norgen's Saliva;Exosome Collection and Preservation Kit (Cat 65400).;No time-consuming ultracentrifugation, filtration nor special syringes required;No precipitation reagents or overnight incubation required;No protease treatment required;Purify pure exosomes that are free from RNA-binding proteins;Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration;RNA can be isolated from the purified exosomes using Norgen's Exosome RNA Purification kit (Cat. 58000).

保存建议

溶液密封室温(15-25°C)保存，未开封至少保存2年。

其他

Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。 目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。