荧光
λex 337 nm; λem 492 nm in chloroform (after derivatization with hexylamine)
一般描述
Dansyl Chloride, also known as 5-dimethylamino naphthalene-1-sulfonyl chloride, is a pre-column derivatization reagent. After the derivatization reaction, the fluorescent derivatives are analyzed using several analytical methods like NMR, IR, UV-vis, and fluorescence microscopy. Dansyl Chloride is a typical aromatic sulphonyl chloride that reacts with various bases, resulting in derivatives with variable stability.
应用
丹磺酰氯(DNSC)是一种伯胺反应剂,广泛应用于氨基酸和肽的N端衍生,以及利用反相HPLC等分析方法进行的多胺检测。 一种用于氨基酸和肽的荧光N-末端衍生化的荧光试剂,并且可用反相HPLC进行检测。
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