一般描述
NHS-dPEG®4-biotin is a water-soluble cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®4-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.
特点和优势
NHS-dPEG ® 4 -生物素被广泛用于标记含有游离胺的分子。单分子量 NHS-dPEG ® 4 -生物素在生物素部分和标记的分子之间有一段长度精确 (19.2 Å) 的长间隔区。该 dPEG ® 垫片使生物素标记物具有高度水溶性。对于生物素化的多肽和蛋白,dPEG ® 间隔物显著减少了修饰分子的聚集和沉淀,减少了分子的非特异性结合,这两者都是已经用广泛使用的 NHS-LC-biotin 生物素化的分子的常见问题。
Sigma-Aldrich西格玛奥德里奇(上海)贸易有限公司
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