多糖多酚/复杂植物RNA快速提取试剂盒

RN53-EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒

产品介绍：

     本公司推出EASYspin无苯酚、氯仿RNA快速提取技术基础上，又成功研发基因组DNA清除柱技术可以有效清除gDNA残留，得到的RNA一般不需要DNase消化，可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶， 离心沉淀去除多糖多酚和次级代谢产物，然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱，然后RNA被选择性洗脱滤过， 吸附在基因组DNA清除柱上的残留DNA无法洗脱连同柱子一起丢弃从而清除掉DNA。滤过的RNA用乙醇调节结合条件后，RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜，再通过一系列快速的漂洗－离心的步骤，去蛋白液和漂洗液将细胞代谢物，蛋白等杂质去除， 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。

产品特点：

1.不使用有毒的苯酚，氯仿等试剂，也不需要乙醇沉淀等步骤。 

2.简捷，单个样品操作一般可在25分钟内完成，世界上简单快速的试剂盒。 

3.成功研发基因组DNA清除柱技术可以有效清除gDNA残留，得到的RNA一般不需要DNase消化，可用于反转录PCR、荧光定量PCR等实验。 

4.适应性广，可以提取包括复杂中草药如石斛/丹参/雪莲/人参、复杂淀粉种子如水稻/小麦/玉米种子、复杂果实如葡萄/蓝莓/草莓/西瓜果实、复杂抗逆植物如冬青/松针/沙棘/胡杨、复杂花卉月季/玫瑰/梅/牡丹花、复杂多糖植物紫菜/仙人掌/芦荟水稻种子等数百种国内外试剂盒提取失败的样品，使用EASYspin系列植物RNA提取试剂盒累计发表文章超过200篇。详细样品列表请参考公司主页产品介绍或者找我们索取200多篇发表文章原文。 

5.多次柱漂洗确保高纯度，OD260/OD280典型的比值达1.9～2.2，无DNA残留,可用于荧光定量PCR、RT-PCR、芯片、二代测序、Northern-blot等各种实验。

艾德莱糖多酚/复杂植物RNA快速提取试剂盒  发布文献

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