Mouse Anti-MBP tag Monoclonal Antibody
DATASHEET
Host:Mouse
Target Protein:MBP tag
IR:Immunogen Range:
Clonality:Monoclonal
Isotype:IgG
Entrez Gene:1039564
Swiss Prot:P0AEX9
Source:Recombinant MBP Protein of Escherichia coli (strain K12):
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Size:50ul
Concentration:1mg/ml
Applications:WB(1:1000-5000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:.
![image.png image.png](/SupplyImg/2023-08-09/6382717668664387522145773.jpg)
仅供科研使用。不能用于人和动物治疗等其它临床诊断用!
关键字: MBP tag;MBP;MMBP; Maltose Binding Pro;ECK4026;JW3994;MalE;MALE_ECOLI;
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