Rabbit Anti-p21 Polyclonal Antibody
DATASHEET
Host:Rabbit
Target Protein:p21
IR:Immunogen Range:1-164/164
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1026
Swiss Prot:P39689
Source:Recombinant human p21:1-164/164
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Background:This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically.
Size:50ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:20-100)
IHC-F(1:20-100)
Cross Reactive Species:Human
Mouse
Rat
仅供科研使用。不能用于人和动物治疗等其它临床诊断用！
关键字： p21;P21 protein;Activating Fragment ;CAP20;CDK Interacting;CDKI;CDKN1A;CDN1A_HUMAN;
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