中文名:3-氨基-9-乙基咔唑
英文名:3-Amino-9-ethylcarbazole
英文别名:3-AMINO-9-ETHYLCARBAZOLE|132-32-1|9-Ethyl-9H-carbazol-3-amine|3-Amino-N-ethylcarbazole|9H-Carbazol-3-amine, 9-ethyl-|9-Ethylcarbazol-3-amine|Carbazole, 3-amino-9-ethyl-|MFCD00004964|9-ethyl-9H-carbazol-3-ylamine|3-amino-9-ethylcabazole|9-ethyl-3-carbazola
纯度:95%
货号:A105545
包装:25g、5g、1g
Cas号:132-32-1
存储温度:2-8°C储存,充氩
产品介绍:
AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Althoμgh not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .过氧化物酶底物. Usage Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps. ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;). ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well. ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT. ? Rinse thoroμghly with distilled water. If desired, follow with an appropriate aqueous-based counterstain. ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; ) ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations
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关键字: 3-AMINO-9-ETHYLCARBAZOLE;132-32-1;9-Ethyl-9H-carbazol-3-amine;3-Amino-N-ethylcarbazole
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