中文名:核酸酶S1 来源于米曲霉
英文名:Nuclease S1 from Aspergillus oryzae
中文别名:Endonuclease S1
英文别名:Endonuclease S1
纯度:≥100,000-500,000 units/ml
货号:N128634
包装:10ku、50ku
Cas号:37288-25-8
存储温度:-20°C储存
产品介绍:
Product Description
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.
Product Application
Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.
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关键字: Endonuclease S1;Endonuclease S1
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