中文名:牛血浆中的血浆胺氧化酶
英文名:Plasma Amine Oxidase from bovine plasma
中文别名:PAO
英文别名:SSAO;Amine oxidase(copper-containing);Amine:oxygen oxidoreductase(deaminating)
纯度:≥17 Tabor units/mg dry weight
货号:P128541
包装:0.6ku、3ku
Cas号:9001-66-5
存储温度:-20°C储存
产品介绍:
Plasma amine oxidase (PAO) catalyzes the reaction:RCH2NH2 + O2 + H2O → RCHO + NH3 + H2O2. Bovine plasma amine oxidase has a molecular weight of 170 kDa and an optimum pH of 6.2 for spermine and 7.2 for spermidine. Amine oxidases are divided into two classes: the pyridoxal and copper containing enzyme to which plasma amine oxidase belongs, and the FAD-containing amino oxidases. Natural substrates include catecholamines, tryptamine derivatives and other physiologically active amines. Plasma amine oxidase is used in research requiring nitrogen group transfers. The molecule is composed of two identical polypeptide chains. There are two pyridoxal phosphates and two atoms of Cu+ per molecule. Bovine plasma amine oxidase is inhibited by copper chelating agents, many carboxyl reagents such as cuprizone, hydroxylamine and cyanide. Benzoic acid and benzyl alcohol are both non-competitive inhibitors (KI = 30 and 34mM respectively). The assay for determination of amine oxidases employed at Aladdin is essentially that of Tabor et al., JBC, 208, 645, (1954) with the reaction temperature reduced to 25°C.
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