Description
This assay employs the quantitative sandwich enzyme immunoassay technique. A Romiplostim protein has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Anti- Romiplostim antiobody present is bound by the immobilized Romiplostim protein. After washing away any unbound substances, a biotin-labeled Romiplostim protein is added to the wells. Following a wash to remove any unbound protein-biotin reagent, a streptavidin- horseradish peroxidase is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of antibody that anti-Romiplostim protein bound in the initial step.
Catalog No.
KAD76501
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 10%. For unopened kits, all reagents are stored as shown on the label of the reagent bottle, please store the Standard, Detection A, Detection B, and Capture Plate at - 20 ℃, the rest reagents should be store at 4℃.
Detection method
Colorimetric
Precision
Intra-Assay Precision (Precision within an assay): <12%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision.
Sample type
Plasma, Serum
Assay type
Quantitative
Sensitivity
0.025 μg/mL
Range
0.03125 μg/mL - 2 μg/mL
Recovery
80-120%
Shipping
2-8 ℃
Note
For Research Use Only.
关键字： Romiplostim ;Nplate;ELISA ;ELISA Kit;
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