HiScript® II One Step RT-PCR Kit is specifically designed for PCR detection of end-point method by taking RNA as template (such as, RNA virus), which can finish gene special primer (GSP), reverse transcription and PCR reaction in one pipe without extra operation of opening pipe or liquid relief, and thus greatly improve the detection flux and reduce the risk of pollution. After integrating with superior capacity of HiScript® II Reverse Transcriptase and Champagne TaqTM plus DNA polymerase, and coordinating with optimized buffering system, the detection sensitivity of HiScript® II One Step RT-PCR Kit can arrive to 0.1pg of total RNA and a fragment with 12kb can be amplified. Moreover, the kit is supplied by a convenient form of Master Mix. 2 × One Step Mix including optimized buffering system and Dntp, while One Step Enzyme Mix contains HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase with optimized scale.
Shanghai YeHua Biological Technology Co., Ltd.
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