All lanes : Anti-CACNA1A Antibody (Center) at 1:2000 dilution Lane 1: NCI-H1299 whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 282 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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