All lanes : Anti-SSX2 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HT-1080 whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: MDA-MB-231 whole cell lysates Lane 5: MDA-MB-453 whole cell lysates Lane 6: human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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