CD 293 TGE Medium, 1X, Liquid
产品描述（Description）
CD 293 TGE培养基是一种有专利的、化学定义的、无动物来源的培养基，专门用于293细胞的高密度悬浮培养和转染。质粒DNA在适应于该培养基的HEK293细胞中的瞬时表达可以获得高体积产率的靶蛋白。建议将293 Expression MAX-1和Feed X Supplement与BPM BPfectin一起使用，以获得更高的蛋白质表达率。
CD 293 TGE培养基专为以下用途配制：
1. 悬浮液HEK293（293T、293EBNA、293F）瞬时表达
2. HEK 293稳定蛋白表达细胞系的悬浮培养
3. HEK293细胞在生物反应器中的大规模高密度生长
产品特性（Product Features）
1. Support suspension HEK 293(293T, 293EBNA, 293F) transient expression
2. >200% cell growth and expression performance compared competitive alternatives
3. Prepared ready-to-use, with no supplementation required
4. Protein free, animal component free, chemically define and regulatory friendly
5. Extensive support by cell culture expert
产品参数（General Specification）
操作流程（Usage Protocol）
① Before transfection, passage 293 cells with CD 293 TGE Medium for at least 3 round from an adaptation procedure, seeding density greater than 0.5 ×106 cells/ml, cell viability >95%. Culture at 150-180 rpm depending on your orbital shaker design and single cell distribution status.
② Seed 293 cells at 0.5 ×106 cells/ml and transfer to pre-warmed fresh medium, subsequently grow the cells to 1.5 to 2.2 ×106 cells/ml.
③ The day before transfection, dilute the culture with fresh medium to 0.8 to 1.0 ×106 cells/ml and cell density at transfection should range from 1.5 × 106 to 2.0 × 106 cells/ml, provided the doubling time is 24 h and viability is greater than 95%.
④ On the day of transfection, using sterile 150 mM NaCl solution to dilute DNA plasmid and add 293 Expression MAX-1 (Catalog # EXP-711) according to the instruction.
⑤ Add BPfectin (Catalog # TF-1157) at ratio of 3:1 (BPfectin : DNA; volume/weight) to DNA solution mix well with vortex for 3 sec.Note: DNA dosage for transfection is 1 ug per 1 million cells in culture, and volume of DNA-BPfectin complexes is 5%(v/v) of culture. (e.g. 500 μL to 10 mL culture).
⑥ Incubate the complexes at RT for 10 min before transfection.
⑦ Add DNA-BPfectin complexes to cells at 5% volume ratio mix well gently.
⑧ 24 hours post transfection, add Feed X Supplement (Catalog # CF-1116) to culture at 10% volume ratio (e.g. 1 mL to 10mL culture).
⑨ Harvest for purification typically 7 days post transfection or when cell viability drops below 55%.
ACRO培养基用户评价
关键字： 培养基;细胞培养基;TGE琼脂培养基;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。