CD47 [Biotinylated] : SIRP alpha Inhibitor Screening ELISA Kit
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背景(Background)
CD47 is a transmembrane protein, a cell surface glycoprotein molecule belonging to the immunoglobulin superfamily that binds to a variety of proteins, including integrins, thrombospondin 1, and signal regulatory protein-α (SIRPα). In recent years, accumulating data have demonstrated that the CD47-SIRP-α axis is a key immune checkpoint in different cancers, including hematological malignancies, similar to PD-1/PD-L1 in solid tumors. Cd47-sirpα blockade has become a next-generation immune checkpoint blocking strategy for various malignancies after PD-1/PD-L1.
原理(Assay Principles)
This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new CD47 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human CD47 to immobilized human SIRP alpha in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated CD47 protein, a human SIRP alpha protein, an anti-CD47 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human SIRP alpha.
b) Add your molecule of interest to the tests.
c) Add human CD47-Biotin to bind the coated SIRP alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
e) Finally, the half maximal inhibitory concentration (IC50) of your compound to CD47: SIRP alpha binding will be determined by comparing OD readings among different experimental groups.
应用说明(Application)
This kit is developed for screening for inhibitors of human CD47 binding to human SIRP alpha.
It is for research use only.
重构方法(Reconstitution)
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储(Storage)
Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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关键字: CD47 ELISA Kit;CD47试剂盒;CD47 ELISA试剂盒;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。