Human HGF R-coupled Magnetic Beads
背景(Background)
The Human HGF R-coupled Magnetic Beads is produced by coupling biotinylated HGF R protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-HGF R antibody or HGF from your sample with high specificity.
表达区间及表达系统(Source)
Human HGF R Protein is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932(Accession # P08581).
应用说明(Application)
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
The Isotype control (Cat. No. SMB-B01) is sold separately.
重构方法(Reconstitution)
See Certificate of Analysis (CoA) for detailed instruction.
存储(Storage)
Upon receipt, please store the beads at -20°C for 1 year in lyophilized state.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理(Assay Principles)
Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂(Formulation)
Please contact us for detailed information.
Contact us for customized product form or formulation.
关键字: HGF R磁珠;HGF R Beads;Magnetic Beads;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。