RN08-EASYspin 细菌RNA快速提取试剂盒
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产品介绍:
独特的裂解液迅速裂解细胞和灭活细胞RNA酶,然后用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, z后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。
产品特点:
1.离心吸附柱内硅基质膜全部采用进口世界有名公司特制吸附膜,柱与柱之间吸附量差异极小,可重复性好。克服了国产试剂盒膜质量不稳定的弊端。2.不需要使用有毒的苯酚,氯仿等试剂,也不需要乙醇沉淀等步骤。3.快速,简捷,单个样品操作一般可在30分钟内完成。4.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.0,基本无DNA残留,可用于RT-PCR,Northern-blot和各种实验。
使用该产品发表的部分文章:http://www.aidlab.cn/news-show.asp?id=192
EASYspin 细菌RNA提取试剂盒发表文章
Cloning and characterization of bagB and bagC, two co-transcribed genes involved in bagremycin biosynthesis in Streptomyces sp. Tü 4128. Ann Microbiol 2013,63(1), : 167–172
EASYspin Plus细菌RNA提取试剂盒发表文章
Relative significances of pH and substrate starch level to roles of Streptococcus bovis S1 in rumen acidosis. AMB Express 2016, 6:80 DOI: 10.1186/s13568-016-0248-2
Design of Ribosome Binding Sites in Streptomyces coelicolor.Current Proteomics 2017, 14(4): 287-292
Antibacterial activity of gallic acid against Shigella flexneri and its effect on biofilm formation by repressing mdoH gene expression. Food Control 2018, 94: 147-154
Study on the drug resistance of clinical strain of acinetobacter baumannii mediated by TEM-1 beta-lactamases and porin CarO. Tianjin Med J, 2018, 46(3): 246-250
Contribution of the colicin receptor CirA to biofilm formation, antibotic resistance, and pathogenicity of Salmonella Enteritidis. J Basic Microbiol. 2019, 1–10. https://doi.org/10.1002/jobm.201900418
FadR1, a pathway-specific activator of fidaxomicin biosynthesis in Actinoplanes deccanensis Yp-1. Applied Microbiology and Biotechnology 2019, https://doi.org/10.1007/s00253-019-09949-y
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