SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
分子别名(Synonym)
Nucleocapsid protein,NP,Protein N
表达区间及表达系统(Source)
SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C5227) is expressed and secreted by human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1).
Predicted N-terminus: Met 1
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
Nucleocapsid (N) 蛋白是冠状病毒中发现的最丰富的蛋白质。冠状病毒N蛋白是一种高度免疫原性的磷蛋白,对病毒基因组复制和细胞信号通路的调节很重要。它是由一个研究小组在冠状病毒(CoV)感染期间筛查ADP核糖基化蛋白时首次发现的(Grunewald M.E.等人,2017,病毒学;517:62-68)。N蛋白中包含的多种功能活性使其不仅是一种结构蛋白,也是抗病毒治疗开发中的一个有趣靶点。由于N蛋白序列的保守性及其强免疫原性,冠状病毒的N蛋白被选为诊断工具。
关键字: Nucleocapsid protein;NP蛋白;Protein N;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。