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抑制剂
2-亚氨基生物素
2-亚氨基生物素
2-亚氨基生物素|T7206|TargetMol
价格
¥
2430
¥
560
¥
1360
包装
100mg
10mg
50mg
最小起订量
1mg
发货地
上海
更新日期
2024-09-29
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产品详情
中文名称:
2-亚氨基生物素
英文名称:
2-Iminobiotin
CAS:
13395-35-2
品牌:
TargetMol
产地:
美国
保存条件:
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
产品类别:
抑制剂
货号:
T7206
2024-09-29
2-亚氨基生物素
2-Iminobiotin
100mg/2430RMB;10mg/560RMB;50mg/1360RMB
2430
TargetMol
美国
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
抑制剂
Product Introduction
Bioactivity
名称
2-Iminobiotin
描述
2-Iminobiotin (Guanidinobiotin) is a cyclic guanidino analog of biotin that acts as a reversible inhibitor of inducible nitric oxide synthase (iNOS) and neuronal NOS (nNOS; Ki of 21.8 and 37.5 μM for mouse iNOS and rat nNOS, respectively).
细胞实验
In vitro hypoxia was induced for 7 h in IMR-32 cell cultures by using our recently described system with minor modifications.?Enzyme stock solutions (100x) of catalase and glucose oxidase ?were diluted in cell culture medium (DMEM/F12, 1% FCS;?final concentration: 120 and 2 U/ml respectively).?A rapid decrease of partial pressure of oxygen (pO2) to levels below 10 mmHg was achieved by adding the enzymes to glucose containing culture medium.?Also a decline in glucose (<1 g/l) and pH (<7.0) was observed, resembling the clinical characteristics of hypoxic-ischemic injury in vivo.?Hypoxic conditions were confirmed with a tissue oxygen pressure monitor .?After the hypoxic insult, cells were washed twice with PBS and cultures were placed into an incubator at 33.5°C (hypothermia) employing culture medium with (i) solvent (citrate buffer 1%) or (ii) 2-IB at 10, 30, 50, 100, and 300 ng/ml.?To determine the optimal "reperfusion" time, a time-interval curve investigating cell damage (LDH release) was performed.?Analyses of LDH release, ROS generation, hydrogen peroxide release, metabolic activity, cell signaling, apoptosis-related protein expression/activity and expression analysis of 35 human apoptosis-related proteins were performed at different time points post-hypoxia[1]
体外活性
All cultures were subjected to 25 h of hypothermia (33.5°C), and incubated with vehicle or 2-iminobiotin (2-IB) (10, 30, 50, 100, and 300 ng/ml).?Cell morphology was evaluated by brightfield microscopy.?Cell damage was analyzed by LDH assays.?Production of reactive oxygen species (ROS) was measured using fluorometric assays.?Western blotting for PARP, Caspase-3, and the phosphorylated forms of akt and erk1/2 was conducted.?To evaluate early apoptotic events and signaling, cell protein was isolated 4 h post-hypoxia and human apoptosis proteome profiler arrays were performed.?Twenty-five hour after the hypoxic insult, clear morphological signs of cell damage were visible and significant LDH release as well as ROS production were observed even under hypothermic conditions.?Post-hypoxic application of 2-IB (10 and 30 ng/ml) reduced the hypoxia-induced LDH release but not ROS production.?Phosphorylation of erk1/2 was significantly increased after hypoxia, while phosphorylation of akt, protein expression of Caspase-3 and cleavage of PARP were only slightly increased.?Addition of 2-IB did not affect any of the investigated proteins.?Apoptosis proteome profiler arrays performed with cellular protein obtained 4 h after hypoxia revealed that post-hypoxic application of 2-IB resulted in a ≥ 25% down regulation of 10/35 apoptosis-related proteins: Bad, Bax, Bcl-2, cleaved Caspase-3, TRAILR1, TRAILR2, PON2, p21, p27, and phospho Rad17[1].
存储条件
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度
DMSO : 12.5 mg/mL (51.37 mM)
关键字
2 Iminobiotin | Inhibitor | NO Synthase | 2-Iminobiotin | 2-IMINOBIOTIN Hydrobromide | inhibit | Nitric oxide synthases | 2Iminobiotin | NOS
相关产品
Diallyl disulfide | Mifepristone | Dexamethasone acetate | Methylene Blue trihydrate | Ethyl cinnamate | L-Arginine hydrochloride | S-Methylisothiourea sulfate | L-Arginine | L-NAME hydrochloride
关键字:
2-IMINOBIOTIN HYDROBROMIDE|||2-亚氨基生物素|||Guanidinobiotin|TargetMol
公司简介
TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿,致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区,已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库,以及多种类型的生化试剂产品,包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等,此外,我们还建设有CADD(计算机辅助药物设计)研究中心、药理实验室、药化合成平台三大技术中心,全方位满足客户的定制需求。 凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持,我们将有效帮助您缩短研发周期,取得更成功的结果。
成立日期
2013-04-18
(12年)
注册资本
566.265100万人民币
员工人数
100-500人
年营业额
¥ 1亿以上
主营行业
天然产物,生化试剂,分子生物学,分子砌块,生物技术服务
经营模式
贸易,工厂,试剂,定制,服务
TargetMol中国(陶术生物)
VIP
3年
公司成立:
12年
注册资本:
566.265100万人民币
企业类型:
有限责任公司(自然人投资或控股)
主营产品:
小分子抑制剂、药物筛选化合物库、药物筛选等
公司地址:
静安区江场三路238号8楼
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