关键词：嘉美生物，Annexin V，细胞信号转导，各种动物ELISA试剂盒，实验技术服务，重组蛋白，eBioscience，荧光染料，流式细胞
简单介绍：
Fluorescent dyes absorb light at certain wavelengths and in turn emit their fluorescence energy at a higher wavelength. Each dye has a distinct emission spectrum, which can be exploited for multicolor analysis. eBioscience antibodies are available conjugated to a wide variety of fluorochromes.
The tables below lists the properties of the most commonly used fluorochromes in flow cytometry. The eBioscience product number is the prefix used in our catalog numbering system to indicate the corresponding formats of our antibodies. We are continually developing new fluorochrome conjugated antibodies for both flow cytometry and microscopy. Please feel free to contact us at tech@ebioscience.com for more information!
Detected in FL4 on dual laser instruments;, can be used interchangeably with Cy5 or Alexa Fluor® 647
Detected in FL4 on dual laser instruments; can be used interchangeably with Cy5 or APC; photostable, making it a great choice for microscopy, in addition to flow cytometry
Detected in FL4 on dual laser instruments (used interchangeably with APC or Alexa Fluor® 647); good choice for intracellular labeling and microscopy
Detected in appropriate channel based on filter set arrangement; tandem dye, resonance energy transfer from APC molecule to Cy5.5
Although not optimally excited by the He-Ne laser, it does produce a bright signal that can be used as a third dye off this laser; please note that emission wavelength requires a distinct set of filters; most instruments will require a 685 LP mirror and 710/20 filter.
Appropriate channel for detection based on filter set arrangement of the instrument; more photostable then APC-Cy7; tandem dye, resonance energy transfer from APC molecule to eFluorTM 780, APC-Cy7, APC-Alexa Fluor® 750 and APC-eFluorTM 780 may be used interchangeably.
Appropriate channel for detection based on filter set arrangement of the instrument; more photostable then APC-Cy7; tandem dye, resonance energy transfer from APC molecule to Alexa Fluor® 750, dim tandem dye. APC-Cy7, APC-Alexa Fluor® 750 and APC-eFluorTM 780 may be used interchangeably.
Table 2: Fluorescent Dyes for Flow Cytometric Analysis - 633nm Excitation
Excited by the violet laser; eFluorTM 605NCcan be used in combination with eFluorTM450, please make sure to use this conjugate for your brightest antigen/proteins of interest
Excited by the violet laser; eFluorTM 625NCcan be used in combination with eFluorTM450, please make sure to use this conjugate for your brightest antigen/proteins of interest
Excited by the violet laser; eFluorTM 650NCcan be used in combination with eFluorTM450 and eFluorTM 605NC, please make sure to use this conjugate for your brightest antigen/proteins of interest.
Excited by the violet laser; eFluorTM 450 can be used interchangeably with Pacific Blue®, can be used in combination with eFluorTM605NC or eFluorTM 625NC; please make sure to use this conjugate for your brightest antigen/proteins of interest
Extremely dim dye excited by the violet laser; suitable for only the brightest antigens
Excited by the violet laser; Pacific Blue® can be used interchangeably with eFluorTM 450, typically a dimmer fluorochrome; please make sure to use this conjugate for your brightest antigen/proteins of interest
Can be used as second dye excited by the violet laser
Dim and only suitable for densely expressed targets, due to its 551nm emission wavelength the Pacific Orange®dye can be used with Pacific Blue® for multicolor analysis with the violet or UV light source, however eFluorTM 605NC is a better alternative
Table 3: Fluorescent Dyes for Flow Cytometric Analysis - 405nm Excitation
Cy-ChromeTM is a registered trademark of Becton, Dickinson and Company. Tri-ColorTM is a registered trademark of Caltag Laboratories. Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc., for research use only.
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