P6119-2μg
Recombinant Murine VEGF120
2μg
464.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
22339
Q00731
E. coli
121aa
28.4kDa
－
Name
Recombinant Murine VEGF120 (Recombinant Murine Vascular Endothelial Growth Factor120; rMuVEGF120)；Recombinant Mouse VEGF120 ; 重组小鼠血管内皮生长因子120
Synonyms
VAS; Vasculotropin; VEGFA; VPF; MVCD1; vascular endothelial growth factor A; Vascular permeability factor; VEGF-A; VEGFMGC70609; VPFvascular endothelial growth factor
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5ng/ml, corresponding to a specific activity of >2.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rMuVEGF120 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells that stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Mouse express alternately spliced isoforms of 120, 164, 182 amino acids (a.a.) in length. The VEGF120 shares 98%a.a. sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively.
Amino Acid Sequence
MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
包装清单:
P6119-2μg
Recombinant Murine VEGF120
2μg
P6119-10μg
Recombinant Murine VEGF120
10μg
P6119-100μg
Recombinant Murine VEGF120
100μg
P6119-1mg
Recombinant Murine VEGF120
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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