Description:
Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 134 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF.
Source:
Escherichia coli
Unit:
2 μg / 10 μg / 1000 μg
Formulation:
Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.
Molecular Weight:
Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Endotoxin:
Less than 0.1 EU/μg of rRtGDNF as determined by LAL method.
Purity:
> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10 6 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
AA Sequence:
SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV
TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQACC
RPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage:
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is
stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Usage:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes.
FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.
关键字: 大鼠胶质细胞源性神经营养因子(GDNF)(RAT)
赛业(苏州)生物科技有限公司是赛业生物集团2011年在苏州太仓市投资兴建的模式动物生产基地,定位于模式动物的研发与生产。赛业生物2006年成立于美国,在中国设立有赛业苏州公司和赛业广州公司,在北京、上海、武汉、重庆等地均有办事处。目前员工人数达400余名,总规模超22000平方米,业务经营范围涵盖模式动物、分子生物学、细胞生物学、生物信息学等生命科学领域的多个支学科。