描述
The MagMAX™ Plant DNA Isolation Kit is designed for automated high-throughput or manual purification of DNA from a wide variety of plant species. The kit uses MagMAX magnetic bead technology, eliminating the need for phenol/chloroform extraction or alcohol precipitation. The procedures are optimized for the isolation of DNA from 10–100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions.
The MagMAX Plant DNA Isolation Kit relies on magnetic bead technology, enabling reproducible yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of the beads makes the technology ideal for automated high-throughput nucleic acid purification, as well as for manual purification for low sample throughput. The resulting high-quality DNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR, or next-generation sequencing. See bar graph below for typical genomic DNA yields from various sources.
Features of the MagMAX Plant DNA Isolation Kit include:
• Versatile—suitable for most plant types and parts, including woody, polyphenol-rich, and lignified samples
• High DNA yields—compared to traditional CTAB method
• High quality—isolates high integrity DNA, free of inhibitors and RNA contamination
• Flexible—easily adjustable for high-throughput automation platforms and manual processing
Applications
Isolated DNA is ideal for use in common molecular biology procedures, including:
• qPCR
• next-generation sequencing
关键字: MagMAX Plant DNA Isolation Kit
赛默飞世尔科技公司(Thermo Fisher Scientific),简称赛默飞,成立于1956年,总裁兼首席执行官为葛士柏。公司主要业务为制药与生物、制药学术科研、医疗健康、工业与应用市场。产品主要包括分析仪器、实验室设备、试剂、耗材和软件等。