P5849-5μg
Recombinant Rhesus Macaque IL-4
5μg
464.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rhesus Macaque
574281
Q6U8C1
E. coli
129aa
14.9kDa
－
Name
Recombinant Rhesus Macaque IL-4 (Recombinant Rhesus Macaque Interleukin-4; rRhIL-4)；重组恒河猴白细胞介素4
Synonyms
Interleukin-4; IL-4; B-cell stimulatory factor 1; Lymphocyte stimulatory factor 1; IL4
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg.
Physical Appearance
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 5% trehalose.
Endotoxin
Less than 1EU/μg of rRhIL-4 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-4 (IL-4) is a pleiotropic cytokine mainly produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Additionally, It enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rhesus macaque IL-4 shares about 40%~45%a.a. sequence identity with human, mouse, rat IL-4 and they are species-specific in their activities.
Amino Acid Sequence
HNCHIALREI IETLNSLTEQ KTLCTKLTIT DILAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE DFLERLKTIM REKYSKCSS
包装清单:
P5849-5μg
Recombinant Rhesus Macaque IL-4
5μg
P5849-20μg
Recombinant Rhesus Macaque IL-4
20μg
P5849-100μg
Recombinant Rhesus Macaque IL-4
100μg
P5849-1mg
Recombinant Rhesus Macaque IL-4
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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