P6906-20μg
Recombinant Rat MIP-1α/CCL3
20μg
1152.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rat
25542
P50229
E. coli
69aa
7.9kDa
－
Name
Recombinant Rat MIP-1α/CCL3 (Recombinant Rat Macrophage Inflammatory Protein-1α/CCL3; rRtMIP-1α/CCL3)；重组大鼠巨噬细胞炎症蛋白1α/CCL3
Synonyms
LD78a; MIP-1 alpha; C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 10-100ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
Endotoxin
Less than 0.1EU/μg of rRtMIP-1α/CCL3 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant rat MIP-1α is a 7.9kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and mature rat CCL3 shares 74%, 91% and 88% amino acid sequence identity with human, mouse and cotton rat CCL3, respectively.
Amino Acid Sequence
APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA
包装清单:
P6906-5μg
Recombinant Rat MIP-1α/CCL3
5μg
P6906-20μg
Recombinant Rat MIP-1α/CCL3
20μg
P6906-100μg
Recombinant Rat MIP-1α/CCL3
100μg
P6906-1mg
Recombinant Rat MIP-1α/CCL3
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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