P6942-5μg
Recombinant Rat MEC/CCL28
5μg
464.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rat
114492
Q91Y39
E. coli
116aa
13.1kDa
-
Name
Recombinant Rat MEC/CCL28 (Recombinant Rat Mucosae-associated Epithelial Chemokine/CCL28; rRtMEC/CCL28);重组大鼠粘膜相关上皮趋化因子/CCL28
Synonyms
CCL28; MEC; VIC; chemokine (C-C motif) ligand 28; member 28; MGC71902; Protein CCK1
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 5.0-50ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 200mM NaCl.
Endotoxin
Less than 1EU/μg of rRtMEC/CCL28 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The protein signals through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils. CCL28 shares the most homology with CCL27/CTACK.
Amino Acid Sequence
SEAILPIASS CCTEVSHHIP RRLLERVNSC SIQRADGDCD LAAVILHVKR RRICVSPHNP TLKRWMSASE MKNGKENLCP RKKQDSGKDR KGHTPRKHGK HGTRRIHGTH DHEAPR
包装清单:
P6942-5μg
Recombinant Rat MEC/CCL28
5μg
P6942-20μg
Recombinant Rat MEC/CCL28
20μg
P6942-100μg
Recombinant Rat MEC/CCL28
100μg
P6942-1mg
Recombinant Rat MEC/CCL28
1mg
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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