P6920-100μg
Recombinant Rat MCP-3/CCL7
100μg
2750.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rat
287561
Q9QXY8
E. coli
74aa
8.5kDa
－
Name
Recombinant Rat MCP-3/CCL7 (Recombinant Rat Monocyte Chemotactic Protein-3/CCL7; rRtMCP-3/CCL7)；重组大鼠单核细胞趋化蛋白3/CCL7
Synonyms
MARC; MCP-3; C-C motif chemokine 7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rRtMCP-3/CCL7 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Rat CCL7, belonging to the CC chemokine family, is also named as MCP-3, because the sequence comparison suggested that the rat CCL7 is a homologue of the human MCP-3 and is postulated the rat MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic functions for monocytes and eosinophils, but not for neutrophils. Additionally, it can activated NK cells as well as CD4+ and CD8+ T lymphocytes. CCL7 was found to interact with mMP2.
Amino Acid Sequence
QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP
包装清单:
P6920-2μg
Recombinant Rat MCP-3/CCL7
2μg
P6920-10μg
Recombinant Rat MCP-3/CCL7
10μg
P6920-100μg
Recombinant Rat MCP-3/CCL7
100μg
P6920-1mg
Recombinant Rat MCP-3/CCL7
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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