P6357-25μg
Recombinant Rat KGF-2/FGF-10
25μg
1159.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rat
25443
P70492
E. coli
179aa
20kDa
－
Name
Recombinant Rat KGF-2/FGF-10 (Recombinant Rat Keratinocyte Growth Factor-2/FGF-10; rRtKGF-2/FGF-10)；重组大鼠角质细胞生长因子2/成纤维细胞生长因子10
Synonyms
KGF-2; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120ng/ml, corresponding to a specific activity of >8.3×103IU/mg.
Physical Appearance
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, 500mM NaCl, pH7.4, 5% trehalose.
Endotoxin
Less than 1EU/μg of rRtKGF-2/FGF-10 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Rat FGF-10 shares approximately 95% amino acid sequence identity with human FGF-10. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.
Amino Acid Sequence
QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
包装清单:
P6357-5μg
Recombinant Rat KGF-2/FGF-10
5μg
P6357-25μg
Recombinant Rat KGF-2/FGF-10
25μg
P6357-100μg
Recombinant Rat KGF-2/FGF-10
100μg
P6357-1mg
Recombinant Rat KGF-2/FGF-10
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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