P6420-25μg
Recombinant Rat CDNF
25μg
1152.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rat
361276
P0C5I0
E. coli
163aa
18.8kDa
-
Name
Recombinant Rat CDNF (Recombinant Rat Cerebral Dopamine Neurotrophic Factor; rRtCDNF);重组大鼠大脑多巴胺神经营养因子
Synonyms
ARMETL1; arginine-rich, mutated in early stage tumors-like 1; ARMETL1; ARMET-like protein 1; cerebral dopamine neurotrophic factor; Conserved dopamine neurotrophic factor
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5-25μg/ml on a nitrocellulose-coated microplate.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rRtCDNF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is a protein encoded by the CDNF gene and it is widely expressed in neuronal and non-neuronal tissues. The cerebral dopamine neurotrophic factor (CDNF) also is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). By research, CDNF prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons and it might be beneficial for the treatment of parkinson's disease. Recombinant rat CDNF contains 163 amino acid residues and it shares 83% and 87%a.a. sequence identity with human and murine CDNF.
Amino Acid Sequence
QGLEAGVRSR ADCEVCKEFL NRFYNSLLTR GIDFSVDTIE EELISFCADT KGKENRLCYY LGATKDSATK ILGEVTRPMS VHMPTVKICE KLKKMDSQIC ELKYEKKLDL ESVDLWKMRV AELKQILHSW GEECRACAEK HDYVNLIKEL APKYVETRPQ TEL
包装清单:
P6420-5μg
Recombinant Rat CDNF
5μg
P6420-25μg
Recombinant Rat CDNF
25μg
P6420-100μg
Recombinant Rat CDNF
100μg
P6420-1mg
Recombinant Rat CDNF
1mg
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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