P6461-2μg
Recombinant Porcine IL-2
2μg
464.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Porcine
396868
P26891
E. coli
134aa
15.2kDa
－
Name
Recombinant Porcine IL-2 (Recombinant Porcine Interleukin-2; rPoIL-2)；重组猪白细胞介素2
Synonyms
aldesleukin; Aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×106IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rPoIL-2 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Background
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55kDa IL2Rα, 75kDa IL2Rβ, 64kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant porcine IL-2 is a 15.3kDa protein containing 134 amino acid residues and it shares about 72% amino acid sequence identity with mouse, human and rat IL-2. It also shares 60% and 67% acid sequence identity with rhesus macaque and equus caballus IL-2, respectively.
Amino Acid Sequence
APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT
包装清单:
P6461-2μg
Recombinant Porcine IL-2
2μg
P6461-10μg
Recombinant Porcine IL-2
10μg
P6461-100μg
Recombinant Porcine IL-2
100μg
P6461-1mg
Recombinant Porcine IL-2
1mg
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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